Real-Time RT-PCR Prep v1.1
Last updated
Last updated
The Real-Time RT-PCR Prep CDC COVID-19 RT-PCR is part of the CDC COVID-19 RT-PCR protocols that include the following functionality.
Preconfigured CDC COVID-19 RT-PCR protocols that support the preparation of upper and lower respiratory specimens for real-time RT-PCR qualitative detection of nucleic acid from the 2019-nCoV.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol Type = Sample Prep
Next Steps Configuration
Master Step Name = Assay Set Up Sorting (CDC COVID-19 RT-PCR v1.1)
Step Type = No Outputs
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Comment | Multiline Text |
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Derived Sample | RT-qPCR Assay | Text Dropdown | Required Field | Presets
|
Derived Sample | Sample Name | Built-in |
Master Step Name = Thermofisher TaqPath 1-Step RT-qPCR (CDC COVID-19 RT-PCR v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 3
Naming Convention = {InputItemName}-{LIST:N1,N2,P}
Reagent Kits
Thermofisher TaqPath 1-Step RT-qPCR Master Mix, CG
Supplier = Thermofisher
Catalog Number = A15299; A15300
Control Types
HSC
nCoVPC
NTC
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Combined Primer/Probe Mix (uL) | Numeric | Decimal Places Displayed = 2 | |
Nuclease-free Water (uL) | Numeric | Decimal Places Displayed = 2 | |
TaqPath 1-Step RT-qPCR Master Mix (4x) (uL) | Numeric | Decimal Places Displayed = 2 | |
Total Sample | Numeric | Default = 0 | |
Total Volume (uL) | Numeric | Decimal Places Displayed = 2 |
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Promega GoTaq Probe 1-Step RT qPCR (CDC COVID-19 RT-PCR v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 3
Naming Convention = {InputItemName}-{LIST:N1,N2,P}
Reagent Kits
Promega GoTaq Probe 1- Step RT-qPCR System
Catalog Number = A6120; A6121
Control Types
HSC
nCoVPC
NTC
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Combined Primer/Probe Mix (uL) | Numeric | Decimal Places Displayed = 2 | |
Go Script RT Mix for 1-Step RT-qPCR (uL) | Numeric | Decimal Places Displayed = 2 | |
GoTaq Probe qPCR Master Mix with dUTP (uL) | Numeric | Decimal Places Displayed = 2 | |
Nuclease-free Water (uL) | Numeric | Decimal Places Displayed = 2 | |
Total Sample | Numeric | Default = 0 | |
Total Volume (uL) | Numeric | Decimal Places Displayed = 2 |
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Quantabio qScript XLT One-Step RT-qPCR Tough Mix (CDC COVID-19 RT-PCR v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 3
Naming Convention = {InputItemName}-{LIST:N1,N2,P}
Reagent Kits
Quantabio qScript XLT One-Step RT-qPCR ToughMix
Catalog Number = 95132-100; 95132-02K; 95132-500
Control Types
HSC
nCoVPC
NTC
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Combined Primer/Probe Mix (uL) | Numeric | Decimal Places Displayed = 2 | |
Nuclease-free Water (uL) | Numeric | Decimal Places Displayed = 2 | |
qScript XLT One-Step RT-qPCR Tough Mix (uL) | Numeric | Decimal Places Displayed = 2 | |
Total Sample | Numeric | Default = 0 | |
Total Volume (uL) | Numeric | Decimal Places Displayed = 2 |
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Quantabio UltraPlex 1-Step ToughMix (4X) (CDC COVID-19 RT-PCR v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 3
Naming Convention = {InputItemName}-{LIST:N1,N2,P}
Reagent Kits
Quantabio UltraPlex 1-Step ToughMix (4X)
Catalog Number = 95166-100; 95166-500; 95166-01K
Control Types
HSC
nCoVPC
NTC
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Placement Pattern = Row
Destination Containers
96 well plate
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Combined Primer/Probe Mix (uL) | Numeric | Decimal Places Displayed = 2 | |
Nuclease-free Water (uL) | Numeric | Decimal Places Displayed = 2 | |
Total Sample | Numeric | Default = 0 | |
Total Volume (uL) | Numeric | Decimal Places Displayed = 2 | |
UltraPlex 1-Step ToughMix (4x) (uL) | Numeric | Decimal Places Displayed = 2 |
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = ABI 7500 Fast DX (CDC COVID-19 RT-PCR v1.1)
Step Type = No Outputs
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Directions | Multiline Text | ||
Template File | Text Dropdown | Required Field | Preset
|
Step File Placeholders
Log - Automatically attached
Instrument Driver File - Automatically attached
Upload Result File - Manually upload
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Ct Value | Text | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Report Results (CDC COVID-19 RT-PCR v1.1)
Step Type = No Outputs