MiSeq i100 On-Prem v1.0.0

The integration includes the following:

• Preconfigured MiSeq i100 Series On-Prem Sequencing v1.0 workflow that maps to lab protocols and instrument runs.

• Preconfigured protocols:

  • Assign Analysis Configuration Template (MiSeq i100 Series On-Prem Sequencing v1.0)

  • Make Bulk Pool (MiSeq i100 Series On-Prem Sequencing v1.0)

  • Dilute and Denature (MiSeq i100 Series On-Prem Sequencing v1.0)

  • Load to Dry Cartridge (MiSeq i100 Series On-Prem Sequencing v1.0)

  • AUTOMATED - Sequencing Run (MiSeq i100 Series On-Prem Sequencing v1.0)

  • AUTOMATED - Analysis Run (MiSeq i100 Series On-Prem Sequencing v1.0)

• Automatic validation of run setup information before sample sheet generation and planned run creation.

• Automated planned run creation on MiSeq i100 Series instrument via Illumina Run Manager. The planned run created will be retrieved by MiSeq i100 Series Control Software and used to start a sequencing run.

• Automated tracking of the MiSeq i100 Series sequencing run status and parsing of run metrics.

• Automated tracking of the MiSeq i100 Series analysis run status, result summary and demultiplexing result files.

• Support for configuration of secondary analysis with library prep kit (LPK) and index adapter kit (IAK) via Analysis Configuration Template for the planned run. The analysis is performed by DRAGEN on-premise.

• (Optional) Preconfigured Library Prep Validation v2.3.5 workflow used for validation purposes only. The workflow contains a single-step protocol that models the library prep workflow required to produce libraries tagged with index sequences. At the end of the step, these libraries are routed to MiSeq i100 Series On-Prem Sequencing v1.0 workflow.