# Release Notes

Last Updated: December 2024

Release Date: December 2024

These release notes describe the key changes to software components for the Clarity LIMS NextSeq 500/550 Integration Package v2.5.0.

## Compatibility

Refer to [Compatibility](https://help.claritylims.illumina.com/compatibility#nextseq-500-550) under Instruments & Integrations.

## New Features & Updates

* The NextSeq 500/550 v1.3 protocol now also generates forward orientation samplesheet that is compatible with NextSeq 500/550 Control Software Version 4.2 and Local Run Manager v4.0 for the following modules:
  * Assembly Analysis
  * DNA Enrichment Analysis
  * GenerateFASTQ Analysis
  * Library QC Analysis
  * 16S Metagenomics Analysis
  * PCR Amplicon
  * Resequencing Analysis
  * DNA Amplicon Analysis
  * RNA Fusion Analysis
  * Small RNA Analysis.

{% hint style="danger" %}
Note that sample sheet generated by the updated workflow is incompatible with NextSeq 500/550 Control Software version prior to v4.2 and Local Run Manager version prior to v4.0.
{% endhint %}

{% hint style="info" %}
Reverse-complement sample sheet generated by NextSeq 500/550 v1.3 protocol is compatible with bcl2fastq2 v2.20.0 analysis software.
{% endhint %}

## Defect Repairs

* Removed unused fields Reagent Cartridge Part # and Flow Cell Version from run report.
* In the run report, Genus LIMS Run ID is replaced with Clarity LIMS Run ID.