NovaSeq 6000 API-based v3.7.0

The integration includes the following features:

• Preconfigured NovaSeq 6000 v3.8 workflow that maps to lab protocols and instrument runs.

• The following preconfigured protocols:

  • Run Format (NovaSeq 6000 v3.8)

  • NovaSeq Standard (NovaSeq 6000 v3.8) (supports the loading of pooled libraries into a library tube)

  • NovaSeq Xp (NovaSeq 6000 v3.8) (supports individual lane loading on the NovaSeq)

  • AUTOMATED - NovaSeq Run (NovaSeq 6000 v3.8)

• Automated generation of v1 sample sheet for use with bcl2fastq2 v2.20 analysis software or v2 sample sheet. This file is automatically uploaded to the instrument through the API.

• Automatic validation of run setup information. This information is used to create the run recipe and initiate the run.

• Automated tracking of the NovaSeq sequencing run and parsing of run statistics into Clarity LIMS, including the following features:

  • Progress and metrics of sequencing run

  • Per-instrument sequencing runs (tracked as part of the Run ID field value)

  • Sequencing run parameters

  • Sequencing run data directory location

  • Real-Time Analysis v3 (RTA3) version and other run-specific information

• [Optional] Preconfigured Library Prep Validation v2.3.4 workflow used for validation purposes only. The workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NovaSeq 6000 v3.8 workflow. For more information, refer to NovaSeq 6000 Integration v3.7.0 User Interaction, Validation and Troubleshooting. The Library Prep Validation v2.3.4 workflow is available in IPP v2.9 package.