MiSeqDx v1.5.0

• MiSeq Operating Software (MOS) v1.1.7

• Real-Time Analysis (RTA) v1.16.18

• MiSeq Reporter Software (MRS) v2.2.31.1

Features to note for this release

The Illumina MiSeqDx Integration Package v1.5 includes support for both MiSeqDx Research mode and Diagnostic mode.

• For Diagnostic mode, this integration package supports the following three kits: CF 139-Variant Assay, CF Clinical Sequencing Assay, and Universal Kit.

• For Research mode, the Illumina MiSeq Integration Package v5.3 is also included.

New features

This release supports the integration between BaseSpace Clarity LIMS and the MiSeqDx instrument. At a high level, this integration includes the following features:

• In CF 139-Variant Assay and Universal Kit workflows, indexes are automatically placed on samples when running the PCR Amplification step in the Library Prep protocol.

• Key metrics are parsed back into LIMS following the completion of primary analysis on the instrument.

• Variant call files are attached to the Variant Calling step in the LIMS, individually for each sample and one combined file for all the samples together.

Defects fixed

None

Known issues

This release contains the following known issues, which are prioritized for a later release:

• Following a sequencing run, user must wait until the run report is automatically generated and attached before finishing the MiSeqDx Run step. If user completes the step before these events occur, they cannot return to the step and regenerate the report.

• The metrics parsed back into the LIMS for %Bases Q30 R1 and R2 are not consistent with Sequencing Analysis Viewer.