NovaSeq 6000 File-based v2.2.0

Version: 2.2 Compatibility:

• BaseSpace Clarity LIMS v4.2 or later, though not compatible with v6.0 and later

• NGS Extensions Package v5.15 or later

• BaseSpaceLIMS-AutomationWorker 0.1.0 (LIMS v4.2) or v1.0 (LIMS v5.0)

Illumina NovaSeq Software Compatibility: NovaSeq Control Software (NVCS) v1.3.0

Features to note for this release

This release of the BaseSpace Clarity LIMS integration with the Illumina NovaSeq 6000 system includes updated configuration to expose the NovaSeq Xp workflow. Users that upgrade to or perform a clean installation of this version will have:

• Run mode flexibility. Sample-based choice of the run mode: NovaSeq Standard using the library tube barcode or NovaSeq Xp using the flow cell loading dock

• An updated workflow with support for:S1*, S2, and S4 flow cells per sample

  • Different applications on the same flow cell

  • Sample and reagent calculations based on flow cell

  • Creation of an output file for use with liquid handling robots

• A run recipe (*.json file) that interacts with the latest NovaSeq 6000 instrument software

• A sample sheet compatible with BaseSpace Sequence Hub and bcl2fastq v2.19

• Automatic tracking of the sequencing run and parsing of run metrics per lane and per flow cell

• S1 flow cell will be released in the near future

Known issues

There are no known issues for this release.