Sample Sheet Generation Issue and CLPA Issues When Samples Have Been Assigned QC Flag Prior to Entering Steps

Last Updated: July 2024

Issue

The following issues will happen if any of the samples in the pools has been assigned QC flag in prior steps when using impacted integrations (see Impact to Customer):

• Sample sheet and planned run generation will fail

• Clarity LIMS Product Analytics (CLPA) payload generation (pooling step only) will fail

• CLPA association of libraries with sequencing run will fail

Impact to Customer

Impacted Integration Packages

• NextSeq1k2k INT v2.4.0 and v2.5.0

• NovaSeq X INT v1.1.0 and v1.2.0

• UPA Script 1.2.0, 1.2.1 and 1.3.0

Scenarios

Integration(s)	Scenario	Impact	Recommendations
NextSeq1k2k INT v2.4.0 and v2.5.0 NovaSeq X INT v1.1.0 and v1.2.0	One or more samples in the pool(s) have been assigned with QC flag before entering these steps: Step with automation to generate sample sheet and planned run	Unable to generate sample sheet and planned run	NS1k2k INT: Downgrade to NS1k2k INT v2.3.1 NovaSeq X INT: No workarounds ⚠️ Recommend to update all affected integration packages to the latest version once available
UPA Script 1.2.0, 1.2.1 and 1.3.0	One or more samples in the pool(s) have been assigned with QC flag before entering these steps: Pooling steps Sequencing run steps	CLPA fails to generate pooling step payload CLPA fails to associate library with sequencing run	No workarounds ⚠️ Recommend to update all affected integration packages to the latest version once available

Patch Release to Fix Issue

• NextSeq1k2k INT v2.5.1

• NovaSeqX INT v1.2.1

• UPA INT v1.3.1