Equalizer v1.1 The Equalizer Workflow AmpliSeq for Illumina Comprehensive Panel v3 is part of the AmpliSeq for Illumina Comprehensive Panel protocols that include the following functionality:
Preconfigured AmpliSeq for Illumina Comprehensive Panel protocol that supports the preparation of up to 96 uniquely indexed libraries of genomic DNA or total RNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol 1: Equalizer Workflow (AmpliSeq for Illumina Comprehensive Panel v3 v1.1)
Protocol Type = Library Prep
Next Steps Configuration
Step 1: Clean Up Library (AmpliSeq for Illumina Comprehensive Panel v3 v1.1)
Master Step Name = Clean Up Library (AmpliSeq for Illumina v1.1)
chevron-right hashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
Step 2: Amplify Library (AmpliSeq for Illumina Comprehensive Panel v3 v1.1)
Master Step Name = Amplify Library (AmpliSeq for Illumina v1.1)
chevron-right hashtag Trigger Location = Record Details
Trigger Style = Automatic upon entry
chevron-right hashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
chevron-right hashtag Trigger Location = Not Used
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Group of Defaults
chevron-right hashtag Thermal Cycler Program = AMP
chevron-right hashtag Thermal Cycler Program = EQUAL
chevron-right hashtag Thermal Cycler Program = AMP_7
Step Data
Group of Defaults = Equalizer Workflow
Step 3: Perform Capture and Clean Up (AmpliSeq for Illumina Comprehensive Panel v3 v1.1)
Master Step Name = Perform Capture and Clean Up (AmpliSeq for Illumina v1.1)
Derived Sample Generation = Fixed, 1
chevron-right hashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Step File Placeholders
Log - Automatically attached
Step 4: Elute Library (AmpliSeq for Illumina Comprehensive Panel v3 v1.1)
Master Step Name = Elute Library (AmpliSeq for Illumina v1.1)
Derived Sample Generation = Fixed, 1
chevron-right hashtag Trigger Location = Record Details
Trigger Style = Automatic upon exit
Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Step Data (Master Step Fields)
