NovaSeq Integration Package v3.6.0 contains modifications to the existing automations from NovaSeq Integration Package v3.5.1. The NovaSeq Sequencing v3.8 workflow and Illumina Preset Protocols (IPP) v2.6 are required for the integration package to work.
If you do not want to upgrade the existing NovaSeq Sequencing workflow to the latest version through IPP, you can update the workflow configuration manually from NovaSeq Integration package v3.5.1 to v3.6.0 by completing the following steps.
For manual upgrades from versions prior to v3.5.1, please refer to manual upgrade guides for those versions.
The key change between NovaSeq 6000 v3.7 workflow and NovaSeq 6000 v3.8 workflow that comes with NovaSeq 6000 Integration v3.6.0 is the addition of Clarity LIMS Product Analytics (CLPA) enabled automation scripts. If CLPA is not required, it is not necessary to perform this manual upgrade.
Add New Automations
From Configuration, select the Automation tab.
Add the automations in the following table:
¹ This automation has -l {compoundOutputFileLuid0} in the log file output index.
² This automation has -l {compoundOutputFileLuid1} in the log file output index.
Modify Routing Script Automation on Define Run Format (NovaSeq 6000 v3.7) Step
From Configuration, select the Automation tab.
Under the Step Automation tab, select the Routing Script automation that is enabled on the Define Run Format (NovaSeq 6000 v3.7) master step.
In the automation name field, change the name to Routing Script & Register Step Completed.
Modify Routing Script Automation on Dilute and Denature (NovaSeq 6000 v3.7) Step
From Configuration, select the Automation tab.
Under the Step Automation tab, select the Routing Script automation that is enabled on the Dilute and Denature (NovaSeq 6000 v3.7) master step.
In the automation name field, change the name to Routing Script & Register Step Completed.
Modify Validate Inputs and Selected Container Automation on Load to Flowcell (NovaSeq 6000 v3.7) Step
From Configuration, select the Automation tab.
Under the Step Automation tab, select the Validate Inputs and Selected Container automation that is enabled on the Load to Flowcell (NovaSeq 6000 v3.7) master step.
In the automation name field, change the name to Validate Inputs and Selected Container & Register Step Started.
Modify Validate Inputs Flowcell Type Automation on Dilute, Denature & ExAmp (NovaSeq 6000 v3.7) Step
From Configuration, select the Automation tab.
Under the Step Automation tab, select the Validate Inputs Flowcell Type automation that is enabled on the Dilute, Denature & ExAmp (NovaSeq 6000 v3.7) master step.
In the automation name field, change the name to Validate Inputs Flowcell Type & Register Step Started.
Modify Validate Single Input Automation on Dilute and Denature (NovaSeq 6000 v3.7) Step
From Configuration, select the Automation tab.
Under the Step Automation tab, select the Validate Single Input automation that is enabled on the Dilute and Denature (NovaSeq 6000 v3.7) master step.
In the automation name field, change the name to Validate Single Input & Register Step Started.
Set Trigger Location and Style for New Automations
From Configuration, select the Lab Work tab.
Change the trigger locations and styles for the automations in the following table:
Define Run Format (NovaSeq 6000 v3.7)
Register Step Started & Register NovaSeq Run & Register NovaSeq Run Association & Register NovaSeq RunStarted
AUTOMATED - NovaSeq Run (NovaSeq 6000 v3.7)
Update the existing command line as follows.
Changes are in red.
