iSeq 100 Run Setup v1.0
Last updated
Last updated
The iSeq 100 Run Setup includes the following functionality.
Preconfigured iSeq 100 Run Setup workflow that supports the preparation of samples for use on the Illumina® iSeq™ 100 Sequencing System provides a targeted approach to next-generation sequencing (NGS).
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Local Run Manager and manual sample sheet generation for use on the iSeq 100.
Protocol Type = Sequencing
Next Steps Configuration
Master Step Name = Dilute Library to 1nM - iSeq 100 v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Resuspension Buffer (RSB)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Dilute Library to Loading Concentration - iSeq 100 v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
Resuspension Buffer (RSB)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Manually uploaded
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Add a PhiX Control (iSeq 100 v1.0)
Step Type = No Outputs
Reagent Kits
PhiX Control v3
Supplier = Illumina
Catalog Number = FC-110-3001
Website = www.illumina.com
Resuspension Buffer (RSB)
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Run Setup - iSeq 100 v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
iSeq 100 i1 Reagent v2
Supplier = Illumina
Catalog Number = 300 cycle single - 20031371; 300 cycle four-pack - 20031374; 300 cycle eight-pack - 20040760
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Step Data (Master Step Fields)
Step File Placeholders
Log - Automatically attached
LRM Sample Sheet - Automatically attached
Manual Sample Sheet - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields