NovaSeq 6000Dx API-based v1.3.0

The integration includes the following features:

• Preconfigured NovaSeqDx v1.2 workflow that maps to lab protocols and instrument runs.

• The following preconfigured protocols:

  • Run Format (NovaSeqDx v1.2)

  • NovaSeqDx Standard (NovaSeqDx v1.2) (supports the loading of pooled libraries into a library tube)

  • NovaSeqDx Xp (NovaSeqDx v1.2) (supports individual lane loading on the NovaSeq)

  • AUTOMATED - NovaSeqDx Run (NovaSeqDx v1.2)

• Automated generation of v2 sample sheet. This file is automatically uploaded to the instrument via the API.

• Automatic validation of run setup information. This information is used to create the run recipe and initiate the run.

• Automated tracking of the NovaSeqDx sequencing run and parsing of run statistics into Clarity LIMS, including the following features:

  • Progress and metrics of sequencing run

  • Per-instrument sequencing runs (tracked as part of the Run ID field value)

  • Sequencing run parameters

  • Sequencing run data directory location

  • Real-Time Analysis v3 (RTA3) version and other run-specific information

  • [Optional] Preconfigured Library Prep Validation v2.3.4 workflow used for validation purposes only. The workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NovaSeqDx v1.2 workflow. For more information, refer to NovaSeq 6000Dx Integration v1.3.0 User Interaction, Validation and Troubleshooting. The Library Prep Validation v2.3.4 workflow is available in the Illumina Preset Protocols (IPP) v2.9 package.