Library Preparation v1.1
Last updated
Last updated
The Library Prep AmpliSeq for Illumina Cancer HotSpot Panel V2 is part of the AmpliSeq for Illumina Cancer HotSpot Panel V2 protocols that include the following functionality:
Preconfigured AmpliSeq for Illumina Cancer HotSpot Panel protocol that supports the preparation of up to 96 uniquely indexed libraries of genomic DNA using the AmpliSeq for Illumina workflow.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Dilute DNA (AmpliSeq for Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
AmpliSeq Library PLUS for Illumina
Supplier = Illumina
Catalog Number = 24 - 20019101; 96 - 200191102; 384 - 200191103
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Desired Concentration (ng/uL) | Numeric |
|
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Derived Sample | Concentration | Numeric | Required Field | Decimal Places Displayed = 2 |
Derived Sample | Conc. Units | Numeric | Required Field | |
Derived Sample | Input Amount (ng) | Numeric | Decimal Places Displayed = 0 | |
Derived Sample | Low TE (uL) | Numeric | Decimal Places Displayed = 2 | |
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Sample Volume (uL) | Numeric | Decimal Places Displayed = 2 | |
Derived Sample | Total Volume (uL) | Numeric | Decimal Places Displayed = 2 |
Master Step Name = Qubit (AmpliSeq for Illumina v1.1)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Criteria 1 - Operator | Text | Default = >= | |
Criteria 1 - Source Data Field | Text | Default = Concentration | |
Criteria 1 - Threshold Value | Numeric | ||
Criteria 2 - Operator | Text | Default = <= | |
Criteria 2 - Source Data Field | Text | Default = Concentration | |
Criteria 2 - Threshold Value | Numeric |
Step File Placeholders
Log - Automatically attached
QC Log File - Automatically attached
QC Result File - Automatically attached
Upload File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Row
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Derived Sample | Concentration | Numeric | Required Field | Decimal Places Displayed = 2 |
Derived Sample | Conc. Units | Text | Required Field | |
Derived Sample | Sample Name | Built-in | ||
Measurement | Concentration | Numeric | Decimal Places Displayed = 2 | |
Measurement | Conc. Units | Text |
Master Step Name = Dilute DNA (AmpliSeq for Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
AmpliSeq Library PLUS for Illumina
Supplier = Illumina
Catalog Number = 24 - 20019101; 96 - 200191102; 384 - 200191103
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Desired Concentration (ng/uL) | Numeric |
|
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Derived Sample | Concentration | Numeric | Required Field | Decimal Places Displayed = 2 |
Derived Sample | Conc. Units | Numeric | Required Field | |
Derived Sample | Input Amount (ng) | Numeric | Decimal Places Displayed = 0 | |
Derived Sample | Low TE (uL) | Numeric | Decimal Places Displayed = 2 | |
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Sample Volume (uL) | Numeric | Decimal Places Displayed = 2 | |
Derived Sample | Total Volume (uL) | Numeric | Decimal Places Displayed = 2 |
Master Step Name = Amplify Targets (AmpliSeq for Cancer Hotspot Illumina v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
AmpliSeq Cancer HotSpot Panel V2 for Illumina
Supplier = Illumina
Catalog Number = 20019161
AmpliSeq Library PLUS for Illumina
Supplier = Illumina
Catalog Number = 24 - 20019101; 96 - 200191102; 384 - 200191103
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
PCR Cycles | Numeric | Decimal Places Displayed = 0 | |
Recommended Prep Input (ng) | Multiline Text | Read Only | Default = This protocol supports 1-100 ng. The recommended input is 10 ng high-quality DNA. |
Thermal Cycler Program | Text | Default = AMP |
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | 5X AmpliSeq Cancer HotSpot v2 Panel (uL) | Numeric |
| |
Derived Sample | 5X AmpliSeq HiFi Mix (uL) | Numeric |
| |
Derived Sample | Concentration | Numeric | Required Field |
|
Derived Sample | Conc. Units | Text | Required Field | |
Derived Sample | Input Amount (ng) | Numeric |
| |
Derived Sample | Nuclease-free water (uL) | Numeric |
| |
Derived Sample | Prep Input Amount (ng) | Numeric | Required Field |
|
Derived Sample | Prep Input Volume (uL) | Numeric |
| |
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Total Volume (uL) | Numeric |
| |
Project | Project Name | Built-in |
Master Step Name = Partially Digested Amplicons (AmpliSeq for Illumina v1.1)
Step Type = No Outputs
Reagent Kits
AmpliSeq Library PLUS for Illumina
Supplier = Illumina
Catalog Number = 24 - 20019101; 96 - 200191102; 384 - 200191103
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table (Column Headers)
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Thermal Cycler Program | Text | Default = FUPA |
Step File Placeholders
Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Ligate Indexes (AmpliSeq for Illumina v1.1)
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
AmpliSeq Library PLUS for Illumina
Supplier = Illumina
Catalog Number = 24 - 20019101; 96 - 200191102; 384 - 200191103
Website = www.illumina.com