TruSight Oncology 500 HT v1.1
Protocol 1: DNA/RNA FFPE Extraction (TruSight Oncology 500 HT v1.1)
Protocol Type = Sample Prep
Next Steps Configuration

Step 1: DNA/RNA FFPE Extraction (TruSight Oncology 500 HT v1.1)
Master Step Name = DNA/RNA FFPE Extraction (TSO 500 HT v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 2
Naming Convention = {LIST:{SubmittedSampleName}_DNA,{SubmittedSampleName}_RNA}
Reagent Kits
QIAGEN AllPrep DNA/RNA FFPE Kit
Supplier = Qiagen
Catalog Number = 80234
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Type
Text Dropdown
Required Field
Custom Entries
Presets
DNA
RNA
Project
Project Name
Built-in
Protocol 2: RNA QC and cDNA Synthesis (TruSight Oncology 500 HT v1.1)
Protocol Type = Library Prep
Next Steps Configuration

Step 1: Qubit RNA QC (TruSight Oncology 500 HT v1.1)
Master Step Name = Qubit RNA QC (TSO 500 v1.1)
Step Type = Standard QC
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Type
Text Dropdown
Required Field
Custom Entries
Presets
DNA
RNA
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text Dropdown
Custom Entries
Presets
>=
<=
=
!=
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration (ng/ul)
Criteria 1 - Threshold Value
Numeric
Default = 4.7
Decimal Places Displayed = 2
Criteria 2 - Operator
Text Dropdown
Custom Entries
Presets
>=
<=
=
!=
Default = >=
Criteria 2 - Source Data Field
Text
Default = Concentration (ng/ul)
Criteria 2 - Threshold Value
Numeric
Decimal Places Displayed = 2
Step File Placeholders
Log File - Automatically attached
Qubit Result File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Column
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Measurement
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Step 2: Denature and Anneal RNA (TruSight Oncology 500 HT v1.1)
Master Step Name = Denature and Anneal RNA (TSO 500 v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSight Oncology 500 HT, Box 1
Supplier = Illumina
Catalog Number = 20007000
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Dilution Concentration (ng/ul)
Numeric
Required Field
Range = 4.7 To 10
Decimal Places Displayed = 1
EPH3 to each well (uL)
Numeric
Default = 8.5
FSM (uL)
Text
RVT (uL)
Text
Thermal Cycler Program
Text Dropdown
Custom Entries
Presets
LQ-RNA
HQ-RNA
Step File Placeholders
Calculation Log File - Automatically attached
NextStep Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Buffer Volume (ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (ul)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Step 3: First Strand cDNA Synthesis (TruSight Oncology 500 HT v1.1)
Master Step Name = First Strand cDNA Synthesis v2.0
Step Type = No Outputs
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Thermal Cycler Program
Text Dropdown
Custom Entries
Presets
Synthesize 1st Strand
1stSS
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 4: Second Strand cDNA Synthesis (TruSight Oncology 500 HT v1.1)
Master Step Name = Second Strand cDNA Synthesis (TruSight Oncology 500 HT v1.1)
Step Type = No Outputs
Reagent Kits
TruSight Oncology 500 HT, Box 1
Supplier = Illumina
Catalog Number = 20007000
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Thermal Cycler Program
Text
Read Only
Default = 2ndSS
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 5: cDNA Clean Up (TruSight Oncology 500 HT v1.1)
Master Step Name = cDNA Clean Up (TSO 500 v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}_cDNA
Reagent Kits
TruSight Oncology 500 HT, Box 4
Supplier = Illumina
Catalog Number = 20007003
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
BIND1 Plate Barcode
Text
80% EtOH Prep Date
Date
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Type
Text Dropdown
Required Field
Custom Entries
Presets
DNA
RNA
Project
Project Name
Built-in
Protocol 3: DNA QC and Fragmentation (TruSight Oncology 500 HT v1.1)
Protocol Type = Library Prep
Next Steps Configuration

Step 1: Qubit DNA QC (TruSight Oncology 500 HT v1.1)
Master Step Name = Qubit DNA QC (TruSight Oncology 500 HT v1.1)
Step Type = Standard QC
Measurement Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Type
Text Dropdown
Required Field
Custom Entries
Presets
DNA
RNA
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Criteria 1 - Operator
Text Dropdown
Presets
>=
<=
=
!=
Default = >=
Criteria 1 - Source Data Field
Text
Default = Concentration (ng/ul)
Criteria 1 - Threshold Value
Numeric
Default = 3.3
Decimal Places Displayed = 2
Criteria 2 - Operator
Text Dropdown
Presets
>=
<=
=
!=
Default = >=
Criteria 2 - Source Data Field
Text
Default = Concentration (ng/ul)
Criteria 2 - Threshold Value
Numeric
Decimal Places Displayed = 2
Step File Placeholders
Log File - Automatically attached
Qubit Result File - Automatically attached
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Column
File Column Options
File Column Display = Hide
File Attachment Method = Auto
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Measurement
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Step 2: Fragment DNA (TruSight Oncology 500 HT v1.1)
Master Step Name = Fragment DNA (TruSight Oncology 500 HT v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}_DNA
Reagent Kits
TruSight Oncology 500 HT, Box 4
Supplier = Illumina
Catalog Number = 20007003
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Concentration (ng/ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Placement = Enabled
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Placement Pattern = Column
Destination Containers
96 well plate
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Dilution Concentration (ng/ul)
Numeric
Required Field
Range = 3.3 To 10
Decimal Places Displayed = 0
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Buffer Volume (ul)
Numeric
Decimal Places Displayed = 2
Derived Sample
Sample Name
Built-in
Derived Sample
Sample Volume (ul)
Numeric
Decimal Places Displayed = 2
Project
Project Name
Built-in
Protocol 4: Library Prep (TruSight Oncology 500 HT v1.1)
Protocol Type = Library Prep
Next Steps Configuration

Step 1: End Repair and A-Tailing (TruSight Oncology 500 HT v1.1)
Master Step Name = End Repair and A-Tailing (TruSight Oncology 500 HT v1.1)
Step Type = No Outputs
Reagent Kits
TruSight Oncology 500 HT, Box 3
Supplier = Illumina
Catalog Number = 20007002
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
ERA1-A (uL)
Numeric
Decimal Places Displayed = 0
ERA1-B (uL)
Numeric
Decimal Places Displayed = 0
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 2: Ligate Adapters (TruSight Oncology 500 HT v1.1)
Master Step Name = Ligate Adapters (TruSight Oncology 500 HT v1.1)
Step Type = No Outputs
Reagent Kits
TruSight Oncology 500 HT, Box 2
Supplier = Illumina
Website = www.illumina.com
TruSight Oncology 500 HT, Box 3
Supplier = Illumina
Catalog Number = 20007002
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 3: Clean Up Ligation (TruSight Oncology 500 HT v1.1)
Master Step Name = Clean Up v2.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSight Oncology 500 HT, Box 4
Supplier = Illumina
Catalog Number = 20007003
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 4: Index PCR (TruSight Oncology 500 HT v1.1)
Master Step Name = Index PCR (TruSight Oncology 500 HT v1.1)
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSight Oncology 500 HT, Box 3
Supplier = Illumina
Catalog Number = 20007002
Website = www.illumina.com
TruSight Oncology 500 HT, Box 5
Supplier = Illumina
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Add Labels
Label Groups
TruSight Oncology 500 HT NovaSeq
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
PCR Program
Text
Read Only
Default = I-PCR
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Reagent Name
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Protocol 5: Hybridization (TruSight Oncology 500 HT v1.1)
Protocol Type = Library Prep
Next Steps Configuration

Step 1: Set Up First Hybridization (TruSight Oncology 500 HT v1.1)
Master Step Name = Hybridize v2.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSight Oncology 500 HT, Box 7
Supplier = Illumina
Catalog Number = 20026144
Website = www.illumina.com
TruSight Oncology 500 HT, Box 8
Supplier = Illumina
Catalog Number = 20026138
Website = www.illumina.com
TruSight Oncology 500 HT, Box 9
Supplier = Illumina
Catalog Number = 20007012
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
PCR Program
Text
Default = HYB1
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 2: Capture Targets One (TruSight Oncology 500 HT v1.1)
Master Step Name = Capture Targets One (TSO 500 v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSight Oncology 500 HT, Box 6
Supplier = Illumina
Catalog Number = 20026143
Website = www.illumina.com
TruSight Oncology 500 HT, Box 7
Supplier = Illumina
Catalog Number = 20026144
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
EE2 (uL)
Numeric
Decimal Places Displayed = 0
HP3 (uL)
Numeric
Decimal Places Displayed = 0
Total Samples
Numeric
Default = 0
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 3: Set Up Second Hybridization (TruSight Oncology 500 HT v1.1)
Master Step Name = Set Up Second Hybridization (TSO 500 v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSight Oncology 500 HT, Box 7
Supplier = Illumina
Catalog Number = 20026144
Website = www.illumina.com
TruSight Oncology 500 HT, Box 8
Supplier = Illumina
Catalog Number = 20026138
Website = www.illumina.com
TruSight Oncology 500 HT, Box 9
Supplier = Illumina
Catalog Number = 20007012
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
PCR Program
Text
Read Only
Default = HYB2
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 4: Capture Targets Two (TruSight Oncology 500 HT v1.1)
Master Step Name = Capture Targets Two (TSO 500 v1.1)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSight Oncology 500 HT, Box 6
Supplier = Illumina
Catalog Number = 20026143
Website = www.illumina.com
TruSight Oncology 500 HT, Box 7
Supplier = Illumina
Catalog Number = 20026144
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
BIND1 Plate Barcode
Text
Comment
Multiline Text
EE2 (uL)
Numeric
Decimal Places Displayed = 0
HP3 (uL)
Numeric
Decimal Places Displayed = 0
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Row
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 5: Amplify Enriched Library (TruSight Oncology 500 HT v1.1)
Master Step Name = Amplify Enriched Library v2.0
Step Type = No Outputs
Reagent Kits
TruSight Oncology 500 HT, Box 7
Supplier = Illumina
Catalog Number = 20026144
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
PCR
Text
Default = EL-PCR
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
Step 6: Clean Up Amplified Enriched Library (TruSight Oncology 500 HT v1.1)
Master Step Name = Clean Up v2.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSight Oncology 500 HT, Box 6
Supplier = Illumina
Catalog Number = 20026143
Website = www.illumina.com
Automations

Queue/Ice Bucket
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Derived Sample
Waiting
Built-in
Expanded View Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
LIMS ID (Container)
Built-in
Project
Project Name
Built-in
Record Details
Step Data (Master Step Fields)
Field Name
Field Type
Options
Additional Options and Dropdown Items
Comment
Multiline Text
80% EtOH Prep Date
Date
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Category
Field Name
Field Type
Options
Additional Options and Dropdown Items
Container
Container Name
Built-in
Container
LIMS ID (Container)
Built-in
Container
Well
Built-in
Derived Sample
Sample Name
Built-in
Project
Project Name
Built-in
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