TruSeq Nano DNA v1.0
Last updated
Last updated
The TruSeq Nano DNA includes the following functionality:
Preconfigured TruSeq Nano DNA protocol that explains how to prepare up to 96 uniquely indexed paired-end libraries of genomic DNA (gDNA) using Illumina® TruSeq® Nano DNA Library Prep kits.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
A routing script that allows sequencing of libraries using any Illumina sequencing instrument.
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Fragment DNA v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {InputItemName}
Reagent Kits
TruSeq Nano DNA LT Library Prep Kit - Set A, Set B
Supplier = Illumina
Catalog Number = Set A: FC-121-4001, Set B: FC-121-4002
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Expanded View Fields
Step Data (Master Step Fields)
Step File Placeholders
Log file - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Repair Ends v1.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSeq Nano DNA LT Library Prep Kit - Set A, Set B
Supplier = Illumina
Catalog Number = Set A: FC-121-4001, Set B: FC-121-4002
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Expanded View Fields
Step Data (Master Step Fields)
Step File Placeholders
Next Step Log - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Master Step Name = Adenylate 3' Ends v2.0
Step Type = No Outputs
Reagent Kits
TruSeq Nano DNA LT Library Prep Kit - Set A, Set B
Supplier = Illumina
Catalog Number = Set A: FC-121-4001, Set B: FC-121-4002
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Expanded View Fields
Step Data (Master Step Fields)
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Ligate Adapters v2.0
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSeq Nano DNA LT Library Prep Kit - Set A, Set B
Supplier = Illumina
Catalog Number = Set A: FC-121-4001, Set B: FC-121-4002
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Expanded View Fields
Label Groups
TruSeq DNA HT
TruSeq DNA LT
Step Data (Master Step Fields)
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = PCR Amplification (TruSeq Nano DNA v1.0.10)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSeq Nano DNA LT Library Prep Kit - Set A, Set B
Supplier = Illumina
Catalog Number = Set A: FC-121-4001, Set B: FC-121-4002
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Expanded View Fields
Step Data (Master Step Fields)
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Collapse
Well Sort Order = Column
Table Columns - Global Fields
Master Step Name = Validate Libraries (TruSeq Nano DNA v1.0.10)
Step Type = Standard QC
Measurement Generation = Fixed, 4
Naming Convention = {SubmittedSampleName}_{OutputItemSubsetNumber} of {OutputItemSubsetTotal}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Expanded View Fields
Step Data (Master Step Fields)
Step File Placeholders
Log File - Manually uploaded
Sample Table
Enable QC Flags = Yes
Sample Display Default = Expand
Well Sort Order = Column
File Column Options
File Column Display = Hide
File Attachment Method = Manual
Table Columns - Global Fields
Master Step Name = Normalize Libraries 2 v2.0.10
Step Type = Standard
Measurement Generation = Fixed, 1
Naming Convention = {InputItemName}
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Expanded View Fields
Step Data (Master Step Fields)
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
The version of PCR Amplification master step name may be different depending on the version of IPP installed.
The version of Validate Libraries master step name may be different depending on the version of IPP installed.
The version of Normalize Libraries 2 master step name may be different depending on the version of IPP installed.
The field value and actual version of the workflows and steps in the routing automation script may be different depending on the version of IPP installed.