TruSeq RNA Exome v1.0
Last updated
Last updated
TruSeq RNA Exome v1.0 includes the following functionality:
Preconfigured TruSeq RNA Exome v1.0 protocol that explains how to convert total RNA into a library of template molecules of known strand origin, then capture the coding regions of the transcriptome using the reagents provided in Illumina® TruSeq® RNA Access Library Prep kits. The resulting library is suitable for subsequent cluster generation and sequencing.
Automated calculation of sample and buffer volumes.
Automated calculation or display of reagents at every step in the protocol.
Automatic step transition when required.
Automatic placement of samples when necessary.
Automated assignment of QC Pass/Fail, based on user-selected threshold values.
Protocol Type = Library Prep
Next Steps Configuration
Master Step Name = Fragment RNA v2.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
TruSeq RNA Library Prep for Enrichment
Supplier = Illumina
Catalog Number = 20020189
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in |
Expanded View Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | LIMS ID (Container) | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Comment | Multiline Text | ||
Desired Diluted Concentration (ng/uL) | Numeric | Required Field | Decimal Places Displayed = 1 |
Thermal Cycler Program | Text | Default = Elution 2 - Frag - Prime |
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Buffer Volume (ul) | Numeric | Decimal Places Displayed = 2 | |
Derived Sample | Concentration | Numeric | Required Field | Decimal Places Displayed = 2 |
Derived Sample | Conc. Units | Text | Required Field | |
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Sample Volume (ul) | Numeric | Decimal Places Displayed = 2 | |
Project | Project Name | Built-in |
Master Step Name = First Strand cDNA Synthesis v2.0
Step Type = No Outputs
Reagent Kits
SuperScript II Reverse Transcriptase
Supplier = Life Technologies
Catalog Number = 18064-014
TruSeq RNA Library Prep for Enrichment
Supplier = Illumina
Catalog Number = 20020189
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Column
Sample Table
Column Headers
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in |
Expanded View Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | LIMS ID (Container) | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Comment | Multiline Text | ||
Thermal Cycler Program | Text Dropdown | Custom Entries |
|
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Second Strand cDNA Synthesis v2.0
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
AMPure XP Beads
Supplier = Beckman Coulter Genomics
Catalog Number = A63881
TruSeq RNA Library Prep for Enrichment
Supplier = Illumina
Catalog Number = 20020189
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in |
Expanded View Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | LIMS ID (Container) | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Comment | Multiline Text | ||
80% EtOH Prep Date | Date |
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Adenylate 3' Ends v2.0
Step Type = No Outputs
Reagent Kits
TruSeq RNA Library Prep for Enrichment
Supplier = Illumina
Catalog Number = 20020189
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in |
Expanded View Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | LIMS ID (Container) | Built-in | ||
Project | Project Name | Built-in |
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
ATL to add to each well (uL) | Numeric | Default = 12.5 | |
Comment | Multiline Text | ||
RSB to add to each well (uL) | Numeric | Default = 2.5 |
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Column
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Ligate Adapters v2.0
Step Type = Add Labels
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits
AMPure XP Beads
Supplier = Beckman Coulter Genomics
Catalog Number = A63881
TruSeq RNA Library Prep for Enrichment
Supplier = Illumina
Catalog Number = 20020189
Website = www.illumina.com
Defaults
Sample Grouping = Group by Containers
Well Sort Order = Row
Sample Table
Column Headers
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Derived Sample | Waiting | Built-in |
Expanded View Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | LIMS ID (Container) | Built-in | ||
Project | Project Name | Built-in |
Label Groups
TruSeq RNA Single Indexes (12 indexes, 24 samples) Set A
TruSeq RNA Single Indexes (12 indexes, 24 samples) Set B
Step Data (Master Step Fields)
Field Name | Field Type | Options | Additional Options and Dropdown Items |
Comment | Multiline Text | ||
Round 1 EtOH to each well (uL) | Numeric | Default = 42 | |
Round 1 RSB to each well (uL) | Numeric | Default = 52.5 | |
Round 2 EtOH to each well (uL) | Numeric | Default = 50 | |
Round 2 RSB to each well (uL) | Numeric | Default = 22.5 | |
1 RSB to add to each well (uL) | Numeric | Default = 2.5 | |
2 LIG to add to each well (uL) | Numeric | Default = 2.5 | |
3 RNA Adapters to add to each well (uL) | Numeric | Default = 2.5 | |
80% EtOH Prep Date | Date |
Step File Placeholders
Log File - Automatically attached
Sample Table
Sample Display Default = Expand
Well Sort Order = Row
Table Columns - Global Fields
Category | Field Name | Field Type | Options | Additional Options and Dropdown Items |
Container | Container Name | Built-in | ||
Container | LIMS ID (Container) | Built-in | ||
Container | Well | Built-in | ||
Derived Sample | Reagent Name | Built-in | ||
Derived Sample | Sample Name | Built-in | ||
Project | Project Name | Built-in |
Master Step Name = Enrich DNA Fragments (TruSeq RNA Exome v1.0.6)
Step Type = Standard
Derived Sample Generation = Fixed, 1
Naming Convention = {SubmittedSampleName}
Reagent Kits