NextSeq 500/550 v2.4.0

The Illumina NextSeq 500/550 Integration v2.4.0 includes the following features and functionality:

• Added support for Oracle Linux. For compatibility, refer to NextSeq 500/550 Integration v2.4.0 Release Notes.

• A new NextSeq 500/550 v1.2 workflow that maps to lab protocols and instrument runs. This workflow includes a minor bug fix and has no additional changes from the previous version.

• Automated generation of the sample sheet. This sample sheet is used with the bcl2fastq2 v2.20.0 analysis software.

• Automated tracking of the NextSeq sequencing runs and parsing of run statistics into Clarity LIMS, which includes the following information:

  • Sequencing run metrics

  • Sequencing run parameters

  • Real-Time Analysis v2 (RTA2) run directory location and other run-specific information

• Preconfigured Library Prep Validation v2.3.3 workflow used for validation purposes. This workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NextSeq 500/550 Sequencing v1.1 workflow. For more information, refer to NextSeq 500/550 Integration v2.4.0 User Interaction, Validation and Troubleshooting.