NextSeq 500/550 v2.5.0
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The Illumina NextSeq 500/550 Integration v2.5.0 includes the following features and functionality:
New workflow that maps to lab protocols and instrument runs. In NextSeq 500/550 Sequencing v1.3, the generated in Dilute and Denature step is now compatible with NextSeq 500/550 Control Software Version 4.2 and Local Run Manager v4.0 for the following modules:
Assembly Analysis
DNA Enrichment Analysis
GenerateFASTQ Analysis
Library QC Analysis
16S Metagenomics Analysis
PCR Amplicon
Resequencing Analysis
DNA Amplicon Analysis
RNA Fusion Analysis
Small RNA Analysis
Automated generation of the sample sheet. The reverse-complement sample sheet is used with the bcl2fastq2 v2.20.0 analysis software.
Automated tracking of the NextSeq sequencing runs and parsing of run statistics into Clarity LIMS, which includes the following information:
Sequencing run metrics
Sequencing run parameters
Real-Time Analysis v2 (RTA2) run directory location and other run-specific information
Preconfigured Library Prep Validation workflow used for validation purposes. This workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NextSeq 500/550 Sequencing workflow. The versions of the Library Prep Validation workflow NextSeq 500/550 Sequencing workflow depends on the version of IPP installed (see table above). For more information, refer to .