> For the complete documentation index, see [llms.txt](https://help.claritylims.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.claritylims.illumina.com/instruments-and-integrations/nextseq550/nextseq550-v2.5.0.md).

# NextSeq 500/550 v2.5.0

The Illumina NextSeq 500/550 Integration v2.5.0 includes the following features and functionality:

* New workflow that maps to lab protocols and instrument runs. In NextSeq 500/550 Sequencing v1.3, the [forward-orientation samplesheet](https://knowledge.illumina.com/software/general/software-general-reference_material-list/000001800) generated in Dilute and Denature step is now compatible with NextSeq 500/550 Control Software Version 4.2 and Local Run Manager v4.0 for the following modules:
  * Assembly Analysis
  * DNA Enrichment Analysis
  * GenerateFASTQ Analysis
  * Library QC Analysis
  * 16S Metagenomics Analysis
  * PCR Amplicon
  * Resequencing Analysis
  * DNA Amplicon Analysis
  * RNA Fusion Analysis
  * Small RNA Analysis
* Automated generation of the sample sheet. The reverse-complement sample sheet is used with the bcl2fastq2 v2.20.0 analysis software.
* Automated tracking of the NextSeq sequencing runs and parsing of run statistics into Clarity LIMS, which includes the following information:
  * Sequencing run metrics
  * Sequencing run parameters
  * Real-Time Analysis v2 (RTA2) run directory location and other run-specific information
* Preconfigured Library Prep Validation workflow used for validation purposes. This workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NextSeq 500/550 Sequencing workflow. The versions of the Library Prep Validation workflow NextSeq 500/550 Sequencing workflow depends on the version of IPP installed (see table above). For more information, refer to [NextSeq 500/550 Integration v2.5.0 User Interaction, Validation and Troubleshooting](/instruments-and-integrations/nextseq550/nextseq550-v2.5.0/nextseq550-v2.5.0-user-interaction-validation-and-troubleshooting.md).


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