NextSeq 500/550 v2.5.0

The Illumina NextSeq 500/550 Integration v2.5.0 includes the following features and functionality:

• New workflow that maps to lab protocols and instrument runs. In NextSeq 500/550 Sequencing v1.3, the forward-orientation samplesheet generated in Dilute and Denature step is now compatible with NextSeq 500/550 Control Software Version 4.2 and Local Run Manager v4.0 for the following modules:

  • Assembly Analysis

  • DNA Enrichment Analysis

  • GenerateFASTQ Analysis

  • Library QC Analysis

  • 16S Metagenomics Analysis

  • PCR Amplicon

  • Resequencing Analysis

  • DNA Amplicon Analysis

  • RNA Fusion Analysis

  • Small RNA Analysis

• Automated generation of the sample sheet. The reverse-complement sample sheet is used with the bcl2fastq2 v2.20.0 analysis software.

• Automated tracking of the NextSeq sequencing runs and parsing of run statistics into Clarity LIMS, which includes the following information:

  • Sequencing run metrics

  • Sequencing run parameters

  • Real-Time Analysis v2 (RTA2) run directory location and other run-specific information

• Preconfigured Library Prep Validation workflow used for validation purposes. This workflow contains a single-step protocol that models the library prep required to produce normalized libraries that are ready for the NextSeq 500/550 Sequencing workflow. The versions of the Library Prep Validation workflow NextSeq 500/550 Sequencing workflow depends on the version of IPP installed (see table above). For more information, refer to NextSeq 500/550 Integration v2.5.0 User Interaction, Validation and Troubleshooting.