NovaSeq X Series v1.3.0

The integration includes the following:

• Preconfigured NovaSeq X Series Sequencing v1.2 workflow that maps to lab protocols and instrument runs.

• Preconfigured protocols:

  • Assign Analysis Configuration Template (NovaSeq X Series Sequencing v1.2)

  • Make Bulk Pool (NovaSeq X Series Sequencing v1.2)

  • Dilute and Denature (NovaSeq X Series Sequencing v1.2)

  • Load to Library Tube Strip (NovaSeq X Series Sequencing v1.2)

  • AUTOMATED - Sequencing Run (NovaSeq X Series Sequencing v1.2)

  • AUTOMATED - Analysis Run (NovaSeq X Series Sequencing v1.2)

• Automatic validation of run setup information before sample sheet generation or planned run creation.

• Automated generation of a samplesheet. The samplesheet will be used to start the sequencing run on NovaSeq X Series Control Software via Local run mode.

• Automated creation of a planned run on Illumina Connected Analytics (ICA). The planned run created will be retrieved by NovaSeq X Series Control Software to start run via Cloud run mode.

• Automated tracking of the NovaSeq X Series sequencing run and parsing of run statistics from BaseSpace Sequence Hub (BSSH) into the LIMS.

• Automated tracking of the NovaSeq X Series analysis run and high level analysis summary from BaseSpace Sequence Hub (BSSH) into the LIMS.

• Support for configuration of multiple secondary analyses with multiple library prep kit (LPK) and index adapter kit (IAK) for the planned run. The analysis are either performed by the ICA in Cloud mode or by DRAGEN on-premise in Local mode.

• (Optional) Preconfigured Library Prep Validation v2.3.5 workflow used for validation purposes only. The workflow contains a single-step protocol that models the library prep workflow required to produce libraries tagged with index sequences. At the end of the step, these libraries are routed to NovaSeq X Series Sequencing v1.2 workflow.

• (Optional) Clarity LIMS Product Analytics (CLPA) integration which makes Clarity data available to the ICA service. CLPA integration installation and configuration is required. Refer to Clarity LIMS Product Analytics Integration v1.4 Configuration for details on configuring and using CLPA.